3FGH

Human mitochondrial transcription factor A box B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-01
Detector	NOIR-1
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	30.280, 99.350, 51.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 1.350
R-factor	0.19077
Rwork	0.188
R-free	0.21403
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.300
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.960
High resolution limit [Å]	1.350
Rmerge	0.049
Number of reflections	17556
Completeness [%]	99.2
Redundancy	6.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	0.1 M NaAcetate, 0.1 M CdCl2 and 21% PEG 4000, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 291K