3FGC
Crystal Structure of the Bacterial Luciferase:Flavin Complex Reveals the Basis of Intersubunit Communication
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | .9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.886, 109.301, 301.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.330 - 2.300 |
| R-factor | 0.18672 |
| Rwork | 0.184 |
| R-free | 0.24087 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1luc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.681 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.330 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.429 | 0.204 |
| Number of reflections | 83194 | |
| <I/σ(I)> | 43.3 | |
| Completeness [%] | 99.9 | 99.08 |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM Na+/K+ phosphate pH 7.5 and 1.65 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
