3FG9
The crystal structure of an universal stress protein UspA family protein from Lactobacillus plantarum WCFS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.355, 118.949, 191.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.600 - 1.470 |
| R-factor | 0.17907 |
| Rwork | 0.177 |
| R-free | 0.21235 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.426 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.600 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.068 | 0.558 |
| Number of reflections | 149737 | |
| <I/σ(I)> | 46.3 | 2.6 |
| Completeness [%] | 99.5 | 92.7 |
| Redundancy | 8.4 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M MgActate. 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
