3FG6
Structure of the C-terminus of Adseverin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.644, 90.384, 98.839 |
| Unit cell angles | 88.79, 88.26, 76.26 |
Refinement procedure
| Resolution | 29.920 - 3.000 |
| R-factor | 0.247 |
| Rwork | 0.244 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p8x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.002 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.040 | 0.142 |
| Number of reflections | 41218 | |
| <I/σ(I)> | 16.784 | |
| Completeness [%] | 90.3 | 85 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG 4000, 200mM sodium isothiocyanate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
