3FG1
Crystal structure of Delta413-417:GS LOX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.38 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.982, 170.224, 104.497 |
| Unit cell angles | 90.00, 95.88, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fnq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.409 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.068 | 0.677 |
| Number of reflections | 303426 | |
| <I/σ(I)> | 21.041 | 2.1 |
| Completeness [%] | 98.8 | 98 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 6-8% PEG 8000, 5% Glycerol, 1% Tween 20, 0.2M CaCl2, 0.1M Imidazole acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
