3FFL

Crystal Structure of the N-terminal Domain of Anaphase-Promoting Complex Subunit 7

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Detector technology	CCD
Collection date	2008-04-02
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97950, 0.97956, 0.97171
Spacegroup name	P 1 21 1
Unit cell lengths	80.493, 64.076, 81.597
Unit cell angles	90.00, 95.23, 90.00

Refinement procedure

Resolution	41.000 - 2.500
R-factor	0.21768
Rwork	0.216
R-free	0.24012
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	0.822
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.500	2.565
High resolution limit [Å]	2.500	2.500
Number of reflections	24411
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	0.2M sodium/potassium tartrate, 15% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	8	294	0.2M sodium/potassium tartrate, 15% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	8	294	0.2M sodium/potassium tartrate, 15% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K