3FF9
Structure of NK cell receptor KLRG1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-20 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.130, 56.930, 68.090 |
| Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| Rwork | 0.223 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1B6E AND 1E87 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.253 |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 29.330 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.057 | 0.032 | 0.343 |
| Total number of observations | 8426 | 6934 | |
| Number of reflections | 24208 | ||
| <I/σ(I)> | 26.1 | 2.8 | |
| Completeness [%] | 97.7 | 99 | 94.7 |
| Redundancy | 3.23 | 3.22 | 2.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | Na,KPO4, pH 8.0, vapor diffusion, temperature 298K |
