3FF0

Crystal structure of a phenazine biosynthesis-related protein (phzb2) from pseudomonas aeruginosa at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.94645,0.97967
Spacegroup name	P 32 2 1
Unit cell lengths	105.540, 105.540, 80.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.502 - 1.900
R-factor	0.156
Rwork	0.155
R-free	0.18100
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.475
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.502	28.502	1.970
High resolution limit [Å]	1.900	4.080	1.900
Rmerge	0.078	0.046	0.807
Number of reflections	41139	8009	8001
<I/σ(I)>	10.74	26.3	1.8
Completeness [%]	99.8	99.6	99.1
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	277	0.8000M K2HPO4, 0.2000M Li2SO4, 1.2000M NaH2PO4, 0.1M CAPS pH 10.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K