3FEW
Structure and Function of Colicin S4, a colicin with a duplicated receptor binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-12-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9761 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 240.340, 240.340, 80.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.450 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.25800 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.334 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.067 | 0.342 |
| Number of reflections | 32515 | |
| <I/σ(I)> | 16.4 | 3.8 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.16M MgCl2, 24% PEG 4000, 20% glycerol, 80mM Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
