3FE0

X-ray crystal structure of wild type human lysozyme in D2O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2001-06-01
Detector	MAC Science DIP-2000
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	33.825, 56.883, 60.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.500
Rwork	0.182
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1REX
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.062	0.251
Number of reflections	18235
<I/σ(I)>	22.409
Completeness [%]	93.9	90.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.3	293	PH 4.30(PD), SMALL TUBES, temperature 293K