3FDO
Structure of human MDMX in complex with high affinity peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.230, 30.950, 50.420 |
| Unit cell angles | 90.00, 124.41, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.400 |
| R-factor | 0.18286 |
| Rwork | 0.179 |
| R-free | 0.24708 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dab |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.304 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 14843 | |
| Completeness [%] | 84.1 | 46.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 290 | 4.3M NaCl, 100mM Hepes, pH7.3, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
