3FDK

Crystal structure of hydrolase DR0930 with promiscuous catalytic activity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 31 2 1
Unit cell lengths	61.505, 61.505, 205.943
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.830 - 2.100
R-factor	0.232
Rwork	0.232
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hzy
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.100
Rmerge	0.041
Number of reflections	26992
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1.3 M ammonium sulfate, 0.1 Tris, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K