3FDJ
The structure of a DegV family protein from Eubacterium eligens.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97942,0.97929 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.445, 57.445, 186.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.910 - 1.800 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.19100 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.374 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.132 | 0.804 |
| Number of reflections | 30039 | |
| <I/σ(I)> | 19.873 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.2M sodium acetate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
