3FDI
Crystal structure of uncharacterized protein from Eubacterium ventriosum ATCC 27560.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.613, 114.932, 59.530 |
| Unit cell angles | 90.00, 128.06, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.200 |
| R-factor | 0.19969 |
| Rwork | 0.198 |
| R-free | 0.24384 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.770 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | 0.570 |
| Number of reflections | 22354 | |
| <I/σ(I)> | 19.73 | 2 |
| Completeness [%] | 98.9 | 89.6 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 0.2 M (NH4)2SO4, Bis-Tris pH5.5, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
