3FC3
Crystal structure of the beta-beta-alpha-Me type II restriction endonuclease Hpy99I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97982 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 90.089, 90.089, 334.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.710 - 1.750 |
| R-factor | 0.18212 |
| Rwork | 0.181 |
| R-free | 0.20533 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.224 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 51564 | |
| <I/σ(I)> | 13.52 | 4.48 |
| Completeness [%] | 96.8 | 99.1 |
| Redundancy | 3.99 | 3.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1 M MES/Sodium hydroxide pH 6.5, 0.1 M Sodium chloride, 0.1 M Lithium sulfate, 30 % PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 10 | 1 | 2 | PEG 400 | ||
| 2 | 1 | 1 | Sodium hydroxide | ||
| 3 | 1 | 1 | Sodium chloride | ||
| 4 | 1 | 1 | Lithium sulfate | ||
| 5 | 1 | 1 | PEG 400 | ||
| 6 | 1 | 2 | MES | ||
| 7 | 1 | 2 | Sodium hydroxide | ||
| 8 | 1 | 2 | Sodium chloride | ||
| 9 | 1 | 2 | Lithium sulfate |
