3FBU
The crystal structure of the acetyltransferase (GNAT family) from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.421, 84.275, 97.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.890 - 1.800 |
| R-factor | 0.19954 |
| Rwork | 0.198 |
| R-free | 0.22352 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.495 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.890 | 1.847 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.105 | 0.743 |
| Number of reflections | 30700 | |
| <I/σ(I)> | 27.56 | 1.89 |
| Completeness [%] | 99.5 | 96 |
| Redundancy | 8.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.1M Mes, 10% PEG10000, 1mM ACoA, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
