3FBI
Structure of the Mediator submodule Med7N/31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.980 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 174.620, 174.620, 117.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.969 - 2.800 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE (2.11) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.618 |
| Number of reflections | 17033 | 5275 |
| <I/σ(I)> | 27.8 | 4.1 |
| Completeness [%] | 99.4 | 98 |
| Redundancy | 11.3 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50mM MES pH 5.6, 1.8M Li2SO4, 10mM MgCl2, vapor diffusion, hanging drop, temperature 293K |
