3FAD
Evaulaution at Atomic Resolution of the Role of Strain in Destabilizing the Temperature Sensitive T4 Lysozyme Mutant Arg96-->His
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.211, 60.211, 97.091 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.700 - 1.200 |
| R-factor | 0.1474 |
| Rwork | 0.147 |
| R-free | 0.17380 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.028 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 1.250 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.057 | 0.330 |
| Number of reflections | 62568 | |
| <I/σ(I)> | 12.83 | 5.3 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 2 M Na/K Phospahte, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
