3FA8
Crystal structure of the apo R132K:Y134F:R111L:L121E mutant of cellular retinoic acid-binding protein II at 1.78 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.610, 37.169, 60.837 |
| Unit cell angles | 106.08, 106.47, 89.97 |
Refinement procedure
| Resolution | 19.420 - 1.780 |
| R-factor | 0.18424 |
| Rwork | 0.179 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g7b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.810 | 1.890 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.056 | 0.181 |
| Number of reflections | 23232 | |
| <I/σ(I)> | 18.2 | 6.7 |
| Completeness [%] | 94.5 | 95.7 |
| Redundancy | 4.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M BTP, 25% PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
