3F9L
Evaulaution at Atomic Resolution of the Role of Strain in Destabilizing the Temperature Sensitive T4 Lysozyme Mutant Arg96-->His
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.82653 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.191, 60.191, 96.743 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.190 |
| R-factor | 0.148 |
| Rwork | 0.152 |
| R-free | 0.18920 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.029 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.240 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.380 | 0.210 |
| Number of reflections | 50477 | |
| <I/σ(I)> | 18.87 | 3.6 |
| Completeness [%] | 89.0 | 88.9 |
| Redundancy | 4.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 2M Na/K Phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
