3F8L
Crystal Structure of the Effector Domain of PhnF from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.558, 78.172, 120.514 |
| Unit cell angles | 90.00, 125.42, 90.00 |
Refinement procedure
| Resolution | 25.100 - 1.900 |
| Rwork | 0.182 |
| R-free | 0.19590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p19 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.597 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.3) |
| Phasing software | BALBES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.280 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 | 0.491 |
| Number of reflections | 68334 | |
| <I/σ(I)> | 8.5 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Micro-seeded into 0.65M ammonium sulfate, 0.1M lithium sulfate, 0.1M tris, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
