3F83
Structure of fusion complex of the minor pilin CfaE and major pilin CfaB of CFA/I pili from ETEC E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.75 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.914, 45.405, 128.473 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.1965 |
| Rwork | 0.193 |
| R-free | 0.23438 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.062 |
| <I/σ(I)> | 23 |
| Completeness [%] | 92.0 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | The CfaEB protein was solubilized in a buffer containing 20 mM MES at pH 6.0 plus 100 mM NaCl. Crystallization was done in hanging drop setup of 1ul of protein solution with 1ul of well solution consisting of 10-11% PEG 8000, 200 mM ammonium sulfate, 100 mM citrate at pH 4.0, VAPOR DIFFUSION |
