3F7P
Crystal structure of a complex between integrin beta4 and plectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.250, 107.250, 203.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.530 - 2.750 |
| R-factor | 0.2066 |
| Rwork | 0.204 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1QG3 and 1MB8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.907 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.530 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 35944 | |
| <I/σ(I)> | 20.4 | 4.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.6 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.8 | 298 | 0.1M Sodium Acetate, 0.2M CaCl2, 10% PEG 6000, pH 5.8, VAPOR DIFFUSION, temperature 298K |
