3F7O
Crystal structure of Cuticle-Degrading Protease from Paecilomyces lilacinus (PL646)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.108, 62.536, 67.562 |
| Unit cell angles | 90.00, 92.76, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3prk |
| RMSD bond length | 1.429 |
| RMSD bond angle | 0.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.136 | 0.564 |
| Number of reflections | 31500 | |
| Completeness [%] | 95.7 | 85.7 |
| Redundancy | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 20% PEG 4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
