3F6L

Structure of the F4 fimbrial chaperone FaeE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 165 mm
Wavelength(s)	0.9364
Spacegroup name	C 1 2 1
Unit cell lengths	109.730, 78.653, 87.842
Unit cell angles	90.00, 96.42, 90.00

Refinement procedure

Resolution	29.876 - 2.801
R-factor	0.257
Rwork	0.255
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f65
RMSD bond length	0.003
RMSD bond angle	0.805
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.122	0.302
Number of reflections	18291
<I/σ(I)>	14.91	7
Completeness [%]	99.8	100
Redundancy	6.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M tris pH 7.5, 50% MPD, 0.2M ammonium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K