3F6J
F17a-G lectin domain with bound GlcNAc(beta1-3)Gal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8047 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 42.801, 42.801, 284.628 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.870 - 1.750 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1O9V chain A |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.316 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.126 | 0.399 |
| Number of reflections | 15865 | |
| <I/σ(I)> | 16.88 | 4.5 |
| Completeness [%] | 94.4 | 90.9 |
| Redundancy | 16.3 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 10%(v/v) 2-propanol, 20%(w/v) PEG 4000, 100mM HEPES, pH 7.5, vapor diffusion, temperature 293K |
