3F6I
Structure of the SeMet labeled F4 fibrial chaperone FaeE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9789, 0.9796, 0.9364 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.285, 75.656, 69.282 |
| Unit cell angles | 90.00, 92.81, 90.00 |
Refinement procedure
| Resolution | 31.473 - 2.788 |
| R-factor | 0.23425 |
| Rwork | 0.231 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.016 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.053 | 0.404 |
| Number of reflections | 19389 | |
| <I/σ(I)> | 23.97 | 4.9 |
| Completeness [%] | 98.6 | 96.4 |
| Redundancy | 7.7 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Tris pH 7.5, 50% MPD, 0.2M ammonium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
