3F65
The F4 fimbrial chaperone FaeE does not self-cap its interactive surfaces
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.689, 78.491, 184.567 |
| Unit cell angles | 90.00, 102.15, 90.00 |
Refinement procedure
| Resolution | 49.853 - 2.290 |
| Rwork | 0.224 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f6i |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.653 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.853 | 2.400 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Rmerge | 0.083 | 0.390 |
| Number of reflections | 122618 | |
| <I/σ(I)> | 14.56 | 3.7 |
| Completeness [%] | 99.5 | 95.6 |
| Redundancy | 5.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
