3F56

The structure of a previously undetected carboxysome shell protein: CsoS1D from Prochlorococcus marinus MED4

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97680, 0.97960
Spacegroup name	P 21 21 21
Unit cell lengths	122.410, 131.286, 131.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.501 - 2.303
R-factor	0.1895
Rwork	0.188
R-free	0.21670
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.217
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.088	0.498
Number of reflections	177663
<I/σ(I)>	16.7	2.9
Completeness [%]		99.9
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	12% Tacsimate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K