3F51

Crystal Structure of the clp gene regulator ClgR from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2007-11-09
Detector	PHILLIPS
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	65.440, 84.820, 71.430
Unit cell angles	90.00, 95.87, 90.00

Refinement procedure

Resolution	45.740 - 2.050
R-factor	0.21359
Rwork	0.212
R-free	0.23801
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.465
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.150
High resolution limit [Å]	2.050	2.050
Number of reflections	48625
<I/σ(I)>	16	2.8
Completeness [%]	99.7	99.8
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	0.1 M sodium chloride, 23% 2-methyl-2,4-pentanediol, 15% glycerol, 0.085 M sodium acetate , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	0.1 M sodium chloride, 23% 2-methyl-2,4-pentanediol, 15% glycerol, 0.085 M sodium acetate , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K