3F4S
Crystal structure of Wolbachia pipientis alpha-DsbA1 T172V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.730, 49.610, 69.000 |
| Unit cell angles | 90.00, 106.33, 90.00 |
Refinement procedure
| Resolution | 66.216 - 1.550 |
| R-factor | 0.155 |
| Rwork | 0.151 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f4r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.886 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.330 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.036 | 0.281 |
| Number of reflections | 31734 | |
| <I/σ(I)> | 15.1 | 2.6 |
| Completeness [%] | 94.9 | 62 |
| Redundancy | 2.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22% PEG 3350, 0.1M Ammonium citrate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
