3F3B
Structure of the phage-like element PBSX protein xkdH from Bacillus Subtilus. Northeast Structural Genomics Consortium target SR352.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-14 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 42.661, 42.661, 283.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.047 | 0.117 |
| Number of reflections | 9878 | |
| <I/σ(I)> | 49.3 | 16.9 |
| Completeness [%] | 98.3 | 96.4 |
| Redundancy | 10 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.55M NaH2PO4, 0.5M KH2PO4, 0.08M Hepes-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
