3F32
Horse spleen apoferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.598, 181.598, 181.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.970 - 1.700 |
| R-factor | 0.17975 |
| Rwork | 0.178 |
| R-free | 0.20871 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1xz1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.236 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | REFMAC (5.4.0074) |
| Refinement software | REFMAC (5.4.0074) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.970 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.102 | 0.615 |
| Number of reflections | 28621 | |
| <I/σ(I)> | 15.5 | 3.6 |
| Completeness [%] | 99.5 | 95.5 |
| Redundancy | 19.58 | 8.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Cadmium sulfate, ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
