3F2L
Crystal structure analysis of the K171A mutation of N-terminal type II cohesin 1 from the cellulosomal ScaB subunit of Acetivibrio cellulolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.934, 53.934, 112.318 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.210 - 1.850 |
| R-factor | 0.14245 |
| Rwork | 0.139 |
| R-free | 0.21218 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1qzn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.097 | 0.490 |
| Number of reflections | 16830 | |
| <I/σ(I)> | 11.4 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293 | 2.4M di-ammonium hydrogen phosphate, 0.1M Tris pH 8.5, Microbatch, temperature 293K |
