3F27
Structure of Sox17 Bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.753, 68.753, 66.329 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.730 - 2.750 |
| R-factor | 0.243 |
| Rwork | 0.242 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gt0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.861 |
| Data scaling software | SAINT |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.330 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.069 | 0.387 |
| Number of reflections | 4970 | |
| <I/σ(I)> | 22.75 | 3.75 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.86 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 30% PEG 3350, 0.2M MGCL2, pH 7.4, vapor diffusion, hanging drop, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 2 | PEG 3350 | ||
| 4 | 1 | 2 | MgCl2 |
