3F1W

Crystal structure of a mutant proliferating cell nuclear antigen that blocks translesion synthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-29
Detector	NOIR-1
Wavelength(s)	1.072
Spacegroup name	P 21 3
Unit cell lengths	123.127, 123.127, 123.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.974 - 2.901
R-factor	0.228
Rwork	0.226
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1plq
RMSD bond length	0.009
RMSD bond angle	1.211
Data reduction software	d*TREK
Data scaling software	d*TREK
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.860	3.000
High resolution limit [Å]	2.900	2.900
Rmerge		0.605
Number of reflections	14067
<I/σ(I)>	14.8	2.5
Completeness [%]	100.0	100
Redundancy	10.8	11.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	291	2.06M ammonium sulfate, 0.1M Sodium citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K