3F1B
The crystal structure of a TetR-like transcriptional regulator from Rhodococcus sp. RHA1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.991, 53.991, 129.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.600 - 2.400 |
| R-factor | 0.22731 |
| Rwork | 0.225 |
| R-free | 0.27839 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.730 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.067 | 0.795 |
| Number of reflections | 8086 | |
| <I/σ(I)> | 48.2 | 2.1 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 7.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2M Ammonium Sulphate, 0.1M HEPES, 2% MPD, 5mM TCEP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
