3F14

Crystal structure of NTF2-like protein of unknown function (YP_680363.1) from CYTOPHAGA HUTCHINSONII ATCC 33406 at 1.45 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97939,0.97925
Spacegroup name	P 32 2 1
Unit cell lengths	57.640, 57.640, 59.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.933 - 1.450
R-factor	0.171
Rwork	0.171
R-free	0.18100
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.753
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.933	25.933	1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.043	0.025	0.538
Number of reflections	20633	3931	3671
<I/σ(I)>	16.58	41.8	2.5
Completeness [%]	99.7	99.4	98.8
Redundancy	7.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2000M NaOAc, 30.0000% PEG-4000, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K