3F0Z

Crystal structure of Clostridium acetobutylicum 8-oxoguanine glycosylase/lyase in its apo-form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-19
Detector	MAR
Wavelength(s)	0.9794, 0.9796
Spacegroup name	P 41 21 2
Unit cell lengths	61.350, 61.350, 158.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.400 - 2.200
R-factor	0.174
Rwork	0.174
R-free	0.21700
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.804	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.102	0.254
Number of reflections	16129
<I/σ(I)>		5.71
Completeness [%]	97.1	92.8
Redundancy	7.69	5.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	14% PEG-4000 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	14% PEG-4000 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K