3F0Y

Crystal structure of the human Adenovirus type 14 fiber knob

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2007-11-11
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 32 1 2
Unit cell lengths	106.470, 106.470, 311.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.400 - 1.800
R-factor	0.18937
Rwork	0.188
R-free	0.21495
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Human Adenovirus type 11 fiber knob
RMSD bond length	0.004
RMSD bond angle	0.926
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.078
Number of reflections	185386
Completeness [%]	99.1	94.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293.15	20% (w/v) PEG 8000, 0.1M CHES, 200mM NaCl, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K