3F0N
Mus Musculus Mevalonate Pyrophosphate Decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 139.619, 139.619, 88.719 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.18369 |
| Rwork | 0.182 |
| R-free | 0.22090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fi4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.922 |
| Number of reflections | 69315 | |
| <I/σ(I)> | 2.37 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 17 MG/ML PROTEIN, 20% PEG 3350, 0.2M AMMONIUM PHOSPHATE, 0.1M SODIUM/POTASSIUM PHOSPHATE BUFFER, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
