3F0M
Crystal structure of radical D105N Synechocystis sp. PcyA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-31 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.940, 95.158, 42.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.560 - 1.500 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.943 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.560 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 46971 | |
| Completeness [%] | 97.5 | 90.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 1.45M Ammonium sulfate, 0.15M NaCl, 0.1M Cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
