3F0I
Arsenate reductase from Vibrio cholerae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-02 |
| Detector | SBC-3 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 118.704, 118.704, 110.013 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.700 - 1.880 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.18900 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.534 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.700 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.090 | 0.764 |
| Number of reflections | 37716 | |
| <I/σ(I)> | 39.832 | 2.04 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 13.7 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 2.4 M sodium malonate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
