3F01
Crystal Structure of Synaptotagmin I C2A domain with Cu(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-05 |
| Detector | SBC-3 |
| Wavelength(s) | 1.379 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.088, 38.561, 44.267 |
| Unit cell angles | 90.00, 97.35, 90.00 |
Refinement procedure
| Resolution | 28.980 - 1.700 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f00 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.351 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.050 | 0.032 | 0.164 |
| Number of reflections | 15627 | ||
| <I/σ(I)> | 54.378 | ||
| Completeness [%] | 99.4 | 96.1 | 97.4 |
| Redundancy | 5.3 | 5.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.0M Li2SO4, 100mM HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 298.0K |
