3F00
Crystal Structure of Synaptotagmin I C2A domain with Cu(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-03 |
| Wavelength(s) | 0.98268 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.647, 38.363, 44.016 |
| Unit cell angles | 90.00, 98.37, 90.00 |
Refinement procedure
| Resolution | 20.600 - 1.360 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rsy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.253 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.410 |
| High resolution limit [Å] | 1.360 | 2.930 | 1.360 |
| Rmerge | 0.165 | 0.105 | 0.514 |
| Number of reflections | 28585 | ||
| <I/σ(I)> | 15.786 | ||
| Completeness [%] | 95.6 | 99.3 | 73.2 |
| Redundancy | 4.1 | 6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.0M Li2SO4, 100mM HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 298.0K, VAPOR DIFFUSION, HANGING DROP |
