3EZX

Structure of Methanosarcina barkeri monomethylamine corrinoid protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	93
Detector technology	CCD
Collection date	2004-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.60296
Spacegroup name	C 2 2 21
Unit cell lengths	67.970, 77.710, 100.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.580 - 2.560
R-factor	0.205
Rwork	0.205
R-free	0.26200
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.560	2.560
Number of reflections	15833
<I/σ(I)>	16.2	3.8
Completeness [%]	95.3	75.9
Redundancy	2.8	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 8000, Magnesium acetate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 8000, Magnesium acetate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 8000, Magnesium acetate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K