3EZO

Crystal structure of acyl-carrier-protein s-malonyltransferase from burkholderia pseudomallei 1710b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2008-09-19
Detector	RIGAKU SATURN 944
Spacegroup name	P 21 21 2
Unit cell lengths	54.920, 117.470, 44.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.130 - 2.050
R-factor	0.158
Rwork	0.155
R-free	0.20800
Structure solution method	MR, MR
Starting model (for MR)	1mla modified with ccp4 chainsaw
RMSD bond length	0.018
RMSD bond angle	1.488
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0046)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.083	0.240
Number of reflections	18128
<I/σ(I)>	19.24	7.6
Completeness [%]	96.4	76.4
Redundancy	6.9	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	290	JCSG+ SCREEN H3: 100MM BISTRIS PH 5.5, 25% PEG 3350, VAPOR DIFFUSION, TEMPERATURE 290K, pH 5.50, VAPOR DIFFUSION, SITTING DROP