3EZ0

Crystal structure of protein of unknown function with ferritin-like fold (YP_832262.1) from Arthrobacter sp. FB24 at 2.33 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-11
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	62.600, 96.710, 71.200
Unit cell angles	90.00, 97.60, 90.00

Refinement procedure

Resolution	29.540 - 2.330
R-factor	0.213
Rwork	0.210
R-free	0.26200
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.304
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.540	29.540	2.410
High resolution limit [Å]	2.330	5.000	2.330
Rmerge	0.067	0.029	0.612
Rmeas	0.083	0.036	0.755
Total number of observations	202485
Number of reflections	35863	7047	6593
<I/σ(I)>	10.67	25.9	1.85
Completeness [%]	97.4	98.1	96.2
Redundancy	5.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.47	293	20.0% polyethylene glycol 3350, 0.171M potassium sodium tartrate, 0.1M MES pH 6.47, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K