3EYS
PFA1 Fab fragment complexed with pyro-Glu3-A-Beta (3-8)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.656, 42.677, 58.630 |
| Unit cell angles | 96.14, 93.83, 91.73 |
Refinement procedure
| Resolution | 25.300 - 1.950 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.25600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.343 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.300 | 2.001 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 23906 | |
| Completeness [%] | 86.4 | 49.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 25% w/v PEG2000-MME, pH 8.5, vapor diffusion, sitting drop, temperature 298K |
