3EYB

Structural and functional insights into the ligand binding domain of a non-duplicated RXR from the invertebrate chordate amphioxus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	200
Detector technology	CCD
Collection date	2004-05-01
Spacegroup name	P 21 21 21
Unit cell lengths	78.723, 96.120, 131.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.790
R-factor	0.202
Rwork	0.202
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1LBD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.890
High resolution limit [Å]	2.790	2.790
Rmerge	0.082
Number of reflections	22831
<I/σ(I)>	5.4	2.5
Completeness [%]	90.3	75
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	1.5 M Na acetate, 0.1 M ADA pH=6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K